Biomarkers Expression
EVs must carry a panel of proteins to classify as exosomes. Alpha Nano Tech provides complete biomarker profiling as an essential step of EV isolates characterization, ensuring at least three exosome relevant biomarker present and negative controls absent in the sample as advised by the International Society of Extracellular Vesicles.
At Alpha Nano Tech we approach EV biomarker profiling with a variety of techniques to help you accurately characterize samples of any complexity.
Our Protein Profiling Services:
• Total protein content - UV rapid test or BCA prtotein content analysis
• fNTA immunophenotyping - quantifying biomarkers expression via fluorescent antibodies labeling and detection
• Westen Blot analysis - capillary Western Blot protein analysis via Jess (Protein Simple)
• LC-MS/MS analysis for in-depth assessment of protein content by orbitrap Exactive Q (Thermo)
• ELISA - quantitative analysis of custom biomarker targets
• Flow cytometry biomarker assessment