Transmission Electron Microscopy

Transmission Electron Microscopy (TEM) allows imaging of smallest objects and structure elements, assessment of size and size distribution. Alpha Nano Tech provides statistically relevant data based on the imaging and analysis of thousands of particles per sample.
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TEM is the best method to cross-validate particle size and size distribution data acquired from other methods, such as Nanoparticle Tracking Analysis (NTA), dynamic light scattering (DLS), or tunable resistive pulse sensing (TRPS).

Immunogold TEM is a direct and powerful tool for the phenotypic characterization of EVs in addition to traditional TEM and protein analysis.

Our SEM technique allows for high-resolution imaging of particles in the sub-micron range. This means that we can study the fine details of your nanoparticles and exosomes down to their morphology and surface characteristics.
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Our EM exosome services:
-Negative staining
-Immunoelectron microscopy
-Size distribution analysis
-Cryo-electron microscopy
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Features
-Qualitative and quantitative analysis
-Highy experienced scientific team
-Consistent reproducible results

Cryo-electron microscopy (Cryo-TEM or Cryo-EM) is another powerful tool to study nanoparticles, including exosomes. The samples are frozen in liquid nitrogen and thus the shape and microstructure are preserved better compared to the traditional TEM method.
Cryo-SEM allows to capture high-resolution, three-dimensional images that are free from artifacts and distortions. This is because cryo-SEM imaging preserves the natural state of your samples, allowing us to capture them in their true form without damaging or altering them in any way.

Our experienced scientists are always available to provide guidance in sample preparation, experimental design, and data analysis.

- unaltered by drying in vacuum imaging, unlike conventional TEM
- fast specimen freeze coverts water into transparent ice crystals
- ice crystal transforms biomolecules in a rigid state allowing high resolution imaging
- sub 10 Å resolution (1 nm) allows imaging near-atomic structures
- quick-fire imaging allows using high power beam up to 200 kV

Alpha Nano Tech provides cryo-TEM imaging service with Thermo Talos Arctica