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Quality Assurance: Nanosight Report Red Flags

July 8, 2016

Growing popularity of the Nanoparticle Tracking Analysis (NTA) and increasing number of service companies and academia core facilities offering Nanosight sample analysis led to a sad conclusion: there are not that many reliable sources and it is not that rare when instrument is misused.

Here are 7 RED FLAGS to include in your reported data check list:

1. At least two files should be reported for each analysis: xls with experiment summary and pdf report summary.

2. Solvent and diluent purity check is reported.

3. Instrument performance is validated by measuring at least one standard with a known size and concentration prior to sample analysis, and the same (or close) camera level and detection threshold are used for the sample analysis.

4. At least 3 replicates of 30 seconds analysis are done for each sample.

5. Sample analysis is based on at least 500 valid tracks.

6. Reported concentration is in the range of 10^7-10^9 particles/mL

7. FILENAME.xls experiment summary state that NO or minor "noise".

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Growing popularity of the Nanoparticle Tracking Analysis (NTA) and increasing number of service companies and academia core facilities offering Nanosi...

Quality Assurance: Nanosight Report Red Flags

July 8, 2016

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