Transition Electron Microscopy (TEM) allows imaging of EVs and assessment of size and size distribution. Alpha Nano Tech provides statistically relevant data based on the imaging and analysis of thousands of particles per sample.

Immunogold TEM is a direct and powerful tool for the phenotypic characterization of EVs in addition to traditional TEM and protein analysis.
 
TEM is the best method to cross-validate particle size and size distribution data acquired from other methods, such as Nanoparticle Tracking Analysis (NTA), dynamic light scattering (DLS), or microfluidic resistive pulse sensing (MRPS). 

Cryo-electron microscopy (Cryo-TEM or Cryo-EM by Talos Arctica, Thermo) is another powerful tool to study nanoparticles, including exosomes. The samples are frozen in liquid propane-ethane mixture and thus the shape and micro-structure are preserved better compared to the traditional TEM method.
Our experienced scientists are always available to provide guidance in sample preparation, experimental design, and data analysis. 
 
• unaltered by drying unlike conventional TEM
• fast specimen freezing coverts water into transparent vitreous ice
• ice preserves biomolecules in a rigid state allowing high resolution imaging
• sub 10 Å resolution (1 nm) allows imaging near-atomic structures
• quick-fire imaging allows using high power beam up to 200 kV