NTA and fNTA
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Nanoparticle Tracking Analysis or NTA - is a rapid and most cited technique for exosome analysis: counts each particle independently to measure size, concentration, size distribution, zeta-potential, percent of fluorescent biomarkers carrying particles, etc.
• Direct analysis in solution
• Detection limit for concentration range starts at 5E+7 particles/mL
• Percent of fluorescent biomarkers carrying particles
Our NTA Services:
• Top-tier models of Nanosight (Malvern) and Zetaview (Particle-Metrix) are available for service
• 405 nm, 488 nm, 520 nm, and 640 nm lasers are available with corresponding long pass cut-off filters for optimal excitation and emission of fluorophores
• EVs purity assessment by membrane labeling
• In-house fluorescent labeling of EVs for fNTA analysis for custom biomarker expression analysis
• Immunophenotyping by multi-color NTA
• Cross-validation by TEM, DLS, Laser diffraction, TRPS, MRPS
• Co-localization of biomarkers and fluorescent labels analysis