NTA and fNTA
NTA is the rapid, most-cited technique for exosome analysis. It counts every particle independently — measuring size, concentration, size distribution, zeta potential, and the fraction carrying your fluorescent biomarker.
NTA illuminates particles suspended in solution with a laser and records the Brownian motion of each one individually. Because every particle is tracked on its own, you get true number-based size distributions and concentrations rather than intensity-weighted estimates. That single-particle resolution is why NTA has become one of the most cited methods for characterizing exosomes and other extracellular vesicles — and why it pairs so well with fluorescent labeling to pull a specific population out of a complex background.
Our NTA Services:
• We run top-tier instruments across all three major NTA platforms and pair them with in-house EV labeling and orthogonal methods — so we can match the technique to your question instead of forcing your sample onto a single box. The Envision (Hyperion Analytical), Nanosight (Malvern Panalytical) and Zetaview (Particle-Metrix) are available for service
• Four excitation lines — 405, 488, 520 and 640 nm — each with a corresponding long-pass cut-off filter for optimal excitation and emission of your fluorophores
• EV purity assessment — membrane labeling to distinguish intact vesicles from co-isolated protein and non-vesicular background
• In-house fluorescent labeling — custom labeling of EVs for fNTA analysis of specific biomarker expression — no need to pre-label your samples
• Multi-color immunophenotyping — multi-color NTA to profile marker-defined subpopulations within a single heterogeneous sample
• Biomarker co-localization — analysis of particles carrying two markers at once, confirming labels reside on the same vesicle
Orthogonal cross-validation
Confirm your NTA results with independent techniques — the kind of orthogonal evidence reviewers and regulators look for:
▪ TEM · DLS · Laser diffraction · TRPS · MRPS
Built for the particles you work with
▪ Exosomes & extracellular vesicles (EVs)
▪ Lipid nanoparticles (LNPs) & drug-delivery carriers
▪ Viral vectors, VLPs & gene-therapy vectors
▪ Vaccine nanoparticles
▪ Polymer & inorganic nanoparticles
▪ Protein aggregates