NTA and fNTA
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The most suitable and most cited technique for exosome analysis: counts each particle independently to measure size, concentration and size distribution.
• Direct analysis in solution
• Low detection limit and wide concentration range: E+7-E+9 particles/mL
• Ability to work in the “Scatter” mode to measure all particles and then analyze the same particles in “Fluorescent” mode to part out and assay EVs labeled with membrane dyes or fluorescently labeled antibodies.
Our NTA Services:
• Both Nanosight and Zetaview instruments are available
• 405 nm, 488 nm, 520 nm, and 640 nm lasers are available with corresponding long pass cut off filters for optimal excitation and emission of fluorophores
• EVs purity assesment by mebrane lavelling
• In house fluorescent labeling of EVs for fNTA analysis for custom biomarker expression analysis
• Immunophenotyping by multicolor NTA
• Cross-validation by TEM, DLS, Laser diffraction, TRPS, MRPS
• Co-localization of biomarkers and fluorescent labels analysis