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Sequencing

RNA/DNA sequencing by NextSeq 1000, Illumina.
Image is a curtsy of Illumina.

Exosomes are small extracellular vesicles that are involved in cell-to-cell communication and have important roles in various physiological and pathological processes. They are known to contain a wide variety of macromolecules, including RNA molecules such as messenger RNA (mRNA), microRNA (miRNA), and long non-coding RNA (lncRNA).

Sequencing RNA from exosomes is a crucial step in understanding their function and the biological processes that they are involved in. This is because the RNA composition of exosomes can vary depending on the cell type, tissue, and physiological or pathological condition. Therefore, sequencing RNA from exosomes can provide insights into the cellular and molecular mechanisms that drive their biogenesis, cargo selection, and uptake by recipient cells.

RNA sequencing also allows for the characterization of specific RNA types, such as miRNA, which are known to have regulatory roles in various cellular processes. The identification of differentially expressed miRNAs in exosomes under different conditions can therefore highlight potential biomarkers of disease or indicators of disease progression.

Moreover, RNA sequencing of exosomes can also shed light on the functional role of exosomes in intercellular communication. For example, exosomes have been shown to deliver functional RNA molecules to recipient cells, which can modulate gene expression and influence cellular behavior. By sequencing RNA from both the exosomes and the recipient cells, it is possible to understand the molecular mechanisms behind this transfer of functional RNA, and how it impacts physiological processes.

Sequencing RNA from exosomes is essential for exosome research as it provides valuable insights into the molecular mechanisms that underpin exosome biogenesis, cargo selection, intercellular communication, and the role of exosomes in health and disease.

Alpha Nano Tech LLC sequencing services:
• RNA or DNA isolation
• Total RNA/DNA content
• RNA/DNA QA/QC
• Next Generation Sequencing with NextSeq 1000 (Illumina)
• Bioinformatics analysis

Features
• Sequence ultra low inputs from small sample quantities
• Consultation and data analysis advise throughout the process
• QC on every step
• Raw data archive download

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