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Nanoparticle Tracking Analysis And Fluorescent Nanoparticle Tracking Analysis

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The most suitable and most cited technique for exosome analysis: counts each particle independently to measure size, concentration and size distribution.

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  • Direct analysis in solution 

  • Low detection limit and wide concentration range: E+7-E+9 particles/mL

  • Ability to work in the “Scatter” mode to measure all particles and then analyze the same particles in “Fluorescent” mode to part out and assay EVs labeled with membrane dyes or fluorescently labeled antibodies.

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Our NTA Services: 

  • Both most advanced Nanosight and Zetaview instruments are available

  • 405 nm, 488 nm, 520 nm, and 640 nm lasers are available with corresponding long pass cut off filters for optimal excitation and emission of fluorophores

  • In house fluorescent labeling of EVs for fNTA analysis

  • Cross-validation by TEM, DLS, Laser diffraction, TRPS, MRPS.

Schematic representation of NTA and fNTA, NTA analysis in scatter and fluorescent modes of liposomes and Exosomes. The measurement is done with the Zetaview NTA instrument. In the fNTA EV membranes were labeled with ExoGlow fluorescent dye (SBI) and the sample is analyzed in both scatter and fluorescent modes. This allows excluding protein aggregates, membrane fragments and other background particles from the analysis. 

Electron microscopy​
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Transition Electron Microscopy (TEM) allows imaging of EVs and assessment of size and size distribution. Alpha Nano Tech provides statistically relevant data based on the imaging and analysis of thousands of particles per sample.

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Immunogold TEM is a direct and powerful tool for the phenotypic characterization of EVs in addition to traditional TEM and protein analysis.

 

TEM is the best method to cross-validate particle size and size distribution data acquired from other methods, such as Nanoparticle Tracking Analysis (NTA), dynamic light scattering (DLS), or tunable resistive pulse sensing (TRPS). 

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Cryo-electron microscopy is another powerful tool to study nanoparticles, including exosomes. The samples are frozen in liquid nitrogen and thus the shape and microstructure are preserved better compared to the traditional TEM method.