alphanano.tech

  • LinkedIn Social Icon
 
Nanoparticle Tracking Analysis And Fluorescent Nanoparticle Tracking Analysis

The most suitable and most cited technique for exosome analysis: counts each particle independently to measure size, concentration and size distribution.

  • Direct analysis in solution 

  • Low detection limit and wide concentration range: E+7-E+9 particles/mL

  • Ability to work in the “Scatter” mode to measure all particles and then analyze the same particles in “Fluorescent” mode to part out and assay EVs labeled with membrane dyes or fluorescently labeled antibodies.

Our NTA Services: 
  • Both most advanced Nanosight and Zetaview instruments are available

  • 405 nm, 488 nm, 520 nm, and 640 nm lasers are available with corresponding long pass cut off filters for optimal excitation and emission of fluorophores

  • In house fluorescent labeling of EVs for fNTA analysis

  • Cross-validation by TEM, DLS, Laser diffraction, TRPS, MRPS.

Schematic representation of NTA and fNTA, NTA analysis in scatter and fluorescent modes of liposomes and Exosomes. The measurement is done with the Zetaview NTA instrument. In the fNTA EV membranes were labeled with ExoGlow fluorescent dye (SBI) and the sample is analyzed in both scatter and fluorescent modes. This allows excluding protein aggregates, membrane fragments and other background particles from the analysis. 

Electron microscopy​

Transition Electron Microscopy (TEM) allows imaging of EVs and assessment of size and size distribution. Alpha Nano Tech provides statistically relevant data based on the imaging and analysis of thousands of particles per sample.

Immunogold TEM is a direct and powerful tool for the phenotypic characterization of EVs in addition to traditional TEM and protein analysis.

 

TEM is the best method to cross-validate particle size and size distribution data acquired from other methods, such as Nanoparticle Tracking Analysis (NTA), dynamic light scattering (DLS), or tunable resistive pulse sensing (TRPS). 

Cryo-electron microscopy is another powerful tool to study nanoparticles, including exosomes. The samples are frozen in liquid nitrogen and thus the shape and microstructure are preserved better compared to the traditional TEM method. 

Our experienced scientists are always available to provide guidance in sample preparation, experimental design, and data analysis. 

 
Our EM exosome services: 
  • Negative staining

  • Immunoelectron microscopy

  • Size distribution analysis

  • Cryo-electron microscopy

Features
  • Qualitative and quantitative analysis

  • Highy experienced scientific team

  • Consistent reproducible results

 
 
Exosomes Isolation Services

Exosomes have been found in virtually all biofluids, including plasma, cerebrospinal fluid (CSF), saliva, urine, conditioned cell culture media, ascites fluid, and tissue lysates. With a wide variety of methods and commercial kits available there is still no universal method to isolate exosomes due to the very different nature of the source biofluids. At Alpha Nano Tech a team of experienced scientists will work closely with you to develop an optimal protocol for your specific project and research interest. We fully understand the advantages and disadvantages of each method and can help you maximize the yield and quality of your exosomes. The exosomes can be shipped back to the client if requested. 

Methods for Exosome Isolation
  • Ultracentrifugation 

  • Size exclusion chromatography

  • Affinity-based purification

  • Ultrafiltration

  • Tangential flow filtration

  • Polymer-based precipitation of exosomes

  • Membrane affinity-based isolation

Features
  • Development and optimization of a custom protocol designed specifically for your unique project

  • Flexible options for each specific case

  • Downstream full spectrum characterization services

  • Fast turnaround time

 
Tunable Resistive Pulse Sensing (TRPS)

TRPS counts and measures particles as they pass a nanopore, as particles cross the pore they briefly increase electrical resistance, creating a resistive pulse, which is precisely proportional to particle volume. TRPS represents a rapid, convenient, accurate and reproducible method for nanoparticle analysis. 

Equipment: qNano (IZON)

 
Exosome RNA Analysis

Exosomal miRNAs are identified as characteristic biomarkers for different types of cancer, including lung, colorectal, ovarian, etc. Exosomal miRNA-based test has been recently approved by the FDA for diagnostics of prostate cancer in patients with ambiguous PSA test results. At Alpha Nano Tech it is our goal to provide tools and expertise to accelerate your exosomal RNA research.

Our RNA Profiling Services:
  • RNA isolation

  • Total RNA content

  • RNA QA/QC

  • Next Generation Sequencing

  • Bioinformatics analysis

Features
  • Sequence ultra low inputs from small sample quantities

  • Consultation and data analysis advise throughout the process

  • RNA QC on every step

  • Fast turnaround time

  • Raw data archive download

 
Exosome Protein Analysis

EVs must carry a panel of proteins to classify as exosomes. Alpha Nano Tech provides complete biomarker profiling as an essential step of EV isolates characterization, ensuring at least three exosome relevant biomarker present and negative controls absent in the sample as advised by the International Society of Extracellular Vesicles. 

 

At Alpha Nano Tech we approach EV biomarker profiling with a variety of techniques to help you accurately characterize samples of any complexity.

Our Protein Profiling Services:
  • Total protein content

  • Traditional and capillary Westen Blot analysis

  • Total EV protein proteomic analysis

  • ELISA 

  • Flow cytometry biomarker assessment

NANO

TECH